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Arbovirus, CSF (Encephalitis)

Mnemonic:    [ARBO CSF]
Name:    Arbovirus, CSF (Encephalitis)
Specimen:    2 mL CSF submitted in a sterile container.
2 forms must be submitted WITH the sample:
1) The health care provider must completely fill out the Human Arboviral Request Form (THIS FORM IS REQUIRED)
2) The lab needs to fill out an HETL Virology Requisition Form

In a sterile container collect CSF at time of onset or within 2-10 days after onset of symptoms.

PHL recommends that a serum sample be sent with the CSF sample.

Specimen screened for:
- West Nile Virus (WNV)
-Eastern Equine Encephalitis (EEE or EEEV)
-St. Louis Encephalitis (SLE)

If negative Powassen PCR and Deer Tick PCR are reflexivity ordered
Minimum Spec:    2 mL
Spec Stability:    Samples should be kept at 4 degrees Celsius (Refrigerated)
Ref Lab/Code:    PHL
  Method:    IgM muliplex assay
Forms are kept in a red folder in Microbiology Dept in the cupboard with the AFB mailers.
Processing Info:    ***Unacceptable conditions: Specimens received without a form. Do Not freeze!***

Turn Around Time: Results should be expected within 5-7 business days of specimen receipt.
Results: IgM multiplexor assay: WNV “Negative or Positive” SLE “Negative” or “Positive”
EEE “Negative” or “Positive”. Comments may include “sent to CDC for confirmation.”

Laboratory confirmed cases of WNV, SLE or EEE must be reported immediately to Maine CDC @ 1.800.821.5821

Arbovirus Specimen Submission Form