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Tacrolimus (Prograf) (FK506) by Tandem Mass Spectrometry

Mnemonic:    [Tacrolimus]
Name:    Tacrolimus (Prograf) (FK506) by Tandem Mass Spectrometry
Specimen:    3 mL whole blood from a Lavender top tube. Pre-dose (trough) levels should be drawn.

Unacceptable samples:
--serum or plasma
-clotted samples
-ambient samples >24 hours old
Minimum Spec:    1 mL
Neonatal Spec:    0.25 mL
Transport Temp:    Refrigerated
Spec Stability:    Stable up to 2 weeks refrigerated.
Ref Lab/Code:    ARUP 0090612
  Method:    Liquid Chromatography/Tandem Mass Spectrometry
Note:    Interpretive Data: Therapeutic range is based on a whole blood specimen drawn 12 hours post-dose or prior to next dose (the trough). Some other factors influencing therapeutic range, dose administered, and result interpretation include time since transplantation, the organ transplanted, co-administration of other immunosuppressants, interaction with other drugs that may increase or decrease the tacrolimus concentration, and the preferences of the transplant centers and clinicians.

Note: Tacrolimus (Prograf®) whole blood concentrations can be measured by either chromatographic or immunoassay methodologies. These two methodologies are not directly interchangeable, and the measured tacrolimus whole blood concentration depends on the type of assay used. Reference ranges may vary according to the specific immunoassay or LC/MS/MS test used. Generally, immunoassays have been reported to have a positive bias relative to the reference assay, HPLC with detection by tandem mass spectrometry (LC/MS/MS).
Processing Info:    If the physician requests that the test be sent to NorDx, order NORDX SO (Unlisted Lab to NorDx).

Send whole blood; do NOT centrifuge.