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HIV-1 Quantitative PCR (HIV-1 Viral Load)

Mnemonic:    [HIV RNA]
Name:    HIV-1 Quantitative PCR (HIV-1 Viral Load)
Specimen:    3 mL plasma from a Lavender (EDTA) top tube. Send to lab ASAP. Collect 2 full 4 mL EDTAs so there is enough plasma to freeze for this assay.

Separate plasma from cells within 24 hours of collection.

Unacceptable Conditions: Serum. Heparinized specimens. Specimens SUBMITTED in plasma preparation tube (aliquot plasma prior to freezing).
Minimum Spec:    1.5 mL
Transport Temp:    FREEZE
Spec Stability:    Stable 6 days refrigerated.
Ref Lab/Code:    ARUP 0055598
  Method:    Real-time Polymerase Chain Reaction (PCR)
Processing Info:    The HIV RNA order will print out with 2 labels so there is a label for each EDTA. Only one aliquot (sample) needs to be frozen to send to the reference lab.