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Brucella Total Antibody, Agglutination

Mnemonic:    [BRUC AB]
Name:    Brucella Total Antibody, Agglutination
Specimen:    1 mL serum from a Red SST or Red top tube. Separate serum from cells ASAP. Hemolyzed, lipemic, heat-inactivated or contaminated specimens are unacceptable.
Minimum Spec:    0.2 mL
Transport Temp:    Refrigerated
Spec Stability:    Stable up to 2 weeks refrigerated.
Ref Lab/Code:    ARUP 0050135
  Method:    Bacterial Agglutination
Note:    Acute and convalescent specimens must be labeled as such; parallel testing is preferred and convalescent samples must be received within 30 days from receipt of the acute specimens. Please mark the specimen plainly as "acute" or "convalescent".

Cross-reactions may occur between Brucella and F. tularensis antigens and antisera, therefore, parallel tests should be run with these antigens. A fourfold rise in titer greater than or equal to 1:160 is considered diagnostic. A single serum titer of 1:160 or 1:320 is suggestive of brucellosis when accompanied by a compatible clinical course in a patient with a history of potential exposures.