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Parasite Examination, Macroscopic (Ectoparasite)

Mnemonic:     
Name:    Parasite Examination, Macroscopic (Ectoparasite)
Specimen:     Immerse the parasite in 5-10 mL 70-80% ethanol and place in a clean, unpreserved container with a tight fitting lid.

If scabies is suspected:
1. Place a drop of mineral oil on a sterile scalpel blade. (Mineral oil is preferred over potassium hydroxide solution or water. Mites will adhere to the oil, skin scales will mix with mineral oil, refractility of differences will be greater between mite and oil, and oil will not dissolve fecal pellets.)

2. Allow some of oil to flow onto the papule
.
3. Scrape vigorously six or seven times to remove the top of the papule. (There should be tiny flecks of blood in the oil.)

4. Transfer the oil and scraped material to a glass slide (can use an applicator stick).

5. Add 1 or 2 extra drops of mineral oil to the slide, and stir the mixture. Any large clumps can be crushed to expose hidden mites.

6. Do NOT send the slides through the peumatic tube system.
Transport Temp:    Store and transport sample at Room Temperature.
Spec Stability:    Stable up to 4 weeks ambient.
  Method:    Macroscopic/Microscopic Examination
Note:    This test is only appropriately ordered for ectoparasite identification (fleas, ticks, lice, worm, scabies etc.).

For body fluid parasitic examinations order a BF OP (Ova & Parasite Exam, Body Fluid

For stool samples order ST OP (Ova & Parasite, Stool).
Processing Info:    This test has been inactivated as an orderable. Send the sample to be identified and manual requisition to Microbiology.

Microbiology: Order as C ADD if TICK ID or WORM ID is not appropriate. ARUP Test code 2007361

Microbiology has mineral oil. It is kept with API supplies in bottom drawer on back wall to the left of the microscope. Send a small container of mineral oil, a cardboard slide container and large coverslips to the collecting location.