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Varicella-Zoster Virus (VZV) and Herpes Simplex Virus (HSV) by DFA

Mnemonic:    [C SOMICRO]
Name:    Varicella-Zoster Virus (VZV) and Herpes Simplex Virus (HSV) by DFA
Specimen:     For Non-Genital sources only---

Call the lab for Viral Transport Media (VTM) before collecting the sample.

LAB: With a sterile pipette remove 1.5 - 2 mL of the media from the vial. This prevents excess sample dilution. Send the VTM to the appropriate nursing unit.

SAMPLE: Vesicle fluid or swab of tissue/skin scrapings placed immediately in Viral Transport Media (VTM).

Gently cleanse the lesion(s) with sterile water or saline. Do not use alcohol as this may inactivate the virus.

Unroof the vesicle using a sterile needle. Moisten the small-tipped swab with VTM and vigorously swab the base fo the lesion, applying enough pressure to collect epithelial cells without causing bleeding.
Transport Temp:    Refrigerated
Ref Lab/Code:    ALI
Note:    Testing is performed at ALI 7 days per/week on the day shift.
Processing Info:    Send to ALI refrigerated. Courier comes twice per day. See courier schedule.

Microbiology tech:
--Fill out an ALI requisition. Found in cupboard with other microbiology sendout forms or in CR. Write in the order "C HSVFA". (Includes the VZV even though it is not in the mnemonic)
--Write the source on the requisition.
--Write it on the CR log.
--Place the sample in the refrigerated ALI box