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Hepatitis B Surface Antigen (HBs Ag)

Mnemonic:    [HepBs Ag]
Name:    Hepatitis B Surface Antigen (HBs Ag)
Specimen:    2 mL serum from a Red SST or Red top tube. Serum is the preferred sample for Hepatitis testing.

Samples should be frozen (-20C or below) if testing will not be completed within 48 hours. Repeated freeze/thaw cycles should be avoided as this can cause analyte deterioration.
Minimum Spec:    2 mL
Transport Temp:    Refrigerated
Spec Stability:    Stable 2 days refrigerated.
Reference Range:    Negative
  Method:    Chemiluminescent Immunoassay
Note:    Hepatitis B Surface antigen confirmation testing will be ordered refexively on all Repeatedly Reactive samples (ARUP # 002128).

If an add-on request is made to a sample >2 days old that has not been frozen, send to ARUP.

The manufacturer’s Instructions For Use document should be reviewed carefully when making exceptions for the use of plasma samples. Heparin and citrate have been shown to lower the signal/cutoff (s/c) values in some HBsAg reactive samples. High negative results (0.80 - 0.99 s/c) obtained on samples collected with these anticoagulants should be interpreted accordingly. Supplemental tests may be required.
Processing Info:    Note: If testing is requested >48 hours after the sample is collected, it can be sent to ARUP as an Unlist Lab. ARUP's order #: 20089 "Hepatitis B Virus Surface Antigen with Reflex to Confirmation ". Sample stability: Serum, 1 week refrigerated.